Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.)
نویسندگان
چکیده
منابع مشابه
Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.)
Polyphenol oxidase from apricot (Prunus armeniaca) (PaPPO) was purified in its latent form (L-PaPPO), and the molecular weight was determined to be 63 kDa by SDS-PAGE. L-PaPPO was activated in the presence of substrate at low pH. The activity was enhanced by CuSO4 and low concentrations (≤ 2 mM) of SDS. PaPPO has its pH and temperature optimum at pH 4.5 and 45 °C for catechol as substrate. It s...
متن کاملMolecular cloning and characterization of apricot fruit polyphenol oxidase.
A reverse transcriptase-polymerase chain reaction experiment was done to synthesize a homologous polyphenol oxidase (PPO) probe from apricot (Prunus armeniaca var Bergeron) fruit. This probe was further used to isolate a full-length PPO cDNA, PA-PPO (accession no. AF020786), from an immature-green fruit cDNA library. PA-PPO is 2070 bp long and contains a single open reading frame encoding a PPO...
متن کاملStamen development and winter dormancy in apricot (Prunus armeniaca).
BACKGROUND AND AIMS In temperate woody perennials, flower bud development is halted during the winter, when the buds enter dormancy. This dormant period is a prerequisite for adequate flowering, is genetically regulated, and plays a clear role in possibly adapting species and cultivars to climatic areas. However, information on the biological events underpinning dormancy is lacking. Stamen deve...
متن کاملAntioxidant and antimicrobial activities of bitter and sweet apricot (Prunus armeniaca L.) kernels.
The present study describes the in vitro antimicrobial and antioxidant activity of methanol and water extracts of sweet and bitter apricot (Prunus armeniaca L.) kernels. The antioxidant properties of apricot kernels were evaluated by determining radical scavenging power, lipid peroxidation inhibition activity and total phenol content measured with a DPPH test, the thiocyanate method and the Fol...
متن کاملOsmotic Drying of Apricot (Prunus armeniaca) in Sucrose Solution
Osmotic drying is a process of partial removal of water by submersing fruits in sucrose solution. Th e goal of this paper is to examine kinetic features of osmotic drying of apricot halves in sucrose solution. For osmotic drying of apricot halves in a thick immovable layer, a planned experiment was conducted. Experimental results were used to create an empirical model of osmotic drying kinetics...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Agricultural and Food Chemistry
سال: 2017
ISSN: 0021-8561,1520-5118
DOI: 10.1021/acs.jafc.7b03210